Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved (1,2). Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including g-secretase presenilin and b-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-b. Caspase-12 is ubiquitously expressed in mouse tissues (1).
Caspase-12 (NT), Casp-12
DescriptionLeft: Western blot analysis of caspase-12 in human (A) and mouse (B) spleen tissue lysates with caspase-12 antibody at 1 μg/ml.
Below: Immunohistochemistry of caspase-12 in human heart tissue with caspase-12 antibody at 10 μg/ml.
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Caspase-12 antibody was raised against a peptide corresponding to amino acids 2 to 17 of murine caspase-12.
Affinity chromatography purified via peptide column
This is a polyclonal antibody.
Caspase-12 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Mouse Caspase-12 (N-Terminus) Peptide (Cat. No. 2325P)
Caspase-12 antibody can be used for detection of caspase-12 by Western blot at 0.5 to 1 μg/ml.
E, WB, IHC
Antibody is supplied in PBS containing 0.02% sodium azide.
Cat. No. 2325P - Caspase-12 Peptide
Caspase-12 antibody can be stored at 4?C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
H, M, R
(NT) A new caspase